• 256 cell-culture chambers
• 1.8 mm x 1.8 mm area per chamber
• < 1 µl working volume per chamber
GRIDs comprise the core of isoCell's automated single-cell cloning workflow. The chambers' small size and optical clarity allows in-chamber verification of single-cell clonality on standard brightfield microscopes directly after plating.
A thin film of cell media (or stem-cell coatings) is reshaped using a fluid microjet of FC40STAR to create an array of 256 chambers on a 6 cm dish. The approach exploits interfacial tension and chambers are separated, throughout the entire workflow, by FC40STAR - an immiscible fluorocarbon.
Your cells are automatically dispensed in nanoliter volumes into the individual GRID chambers. Single cells are clearly visible in the GRID chambers through a standard microscope (4x objective) due to the optical clarity and small chamber size. This enables easy verification of monoclonality immediately after dispensing.
|Physically separated cell environments|
|One-step surface coating|
|Single cells visible at edges|
|Single cells visible directly after plating|
|Low reagent consumption|
Explore our publications which explain the technology in more detail.Download Publications