Single-cell cloning at the microscale
The isoCell introduces a completely new approach to single-cell cloning by harnessing the power and benefits of microscale fluid-shaping.
The isoCell provides high-level automation where it matters, enforces a highly convenient and consistent workflow, and requires almost no training.
It has an incredible small footprint and little weight, making it very portable with almost no space requirement — ideal for any laminar flow hood.
As a multi-user instrument the isoCell stores data and handles several experiments from multiple users simultaneously with ease.
① Create a GRID on 60-mm round-bottom dishes, comprising 256 individual chambers (equiv. to 2½ 96-well plates)
② Automated dispensing of cells into GRID chambers (visualised here by dyed cell-culture medium)
③ Automated feeding of GRID chambers that contain single cells (specified by user via convenient GRID coordinates)
④ Automated retrieval of colonies for downstream workflows
Sideview of a GRID; three chambers were selectively filled with varying amounts of dyed cell-culture medium (orange)
Phase-contrast image of six chambers of a GRID. Image was taken after filling the chambers with cell-culture medium
How many chambers comprise a GRID?
What is the surface area of a GRID-chamber in mm2?
How much volume is used for single-cell cloning per chamber?
How much do I save on consumables when using GRIDs?
Get in touch if you want to learn more about the isoCell and/or request a demo
The isoCell is currently available through iotaSciences Early Access Program
If you want to learn more about the isoCell or request a demo of the unit, please get in touch here