Single-cell isolation and cloning — the establishment of viable and clonal cells derived from a single-cell —
is a highly demanding and labour-intense process. It is however at the heart of many fields in cell-biology — e.g., cell-line engineering, CRISPR-Cas9 genome-editing, the analyses of cell-to-cell heterogeneity
and many more.
In particular, the widespread adoption of genome-editing in conjunction with iPSCs is driving the demand for automation and miniaturization of single-cell isolation, feeding, and clone picking.
The isoCell offers a '3-in-1' benchtop solution that makes isolating and cloning single-cells easy by harnessing iotaSciences' unique fluid-shaping technology.
"For our line of research, we need to generate many different gene edited clonal (stem) cell lines. However, isolating and expanding single cells is a very long and labour intensive process that can take up significant resources. IotaSciences’ isoCell allows us to bypass these issues and facilitates the generation of clonal (stem) cell lines with minimal hands-on time while considerably reducing the amount of consumables. I am very impressed with the excellent optics of the system that requires nothing more than a standard microscope. In our hands, the isoCell is the ideal tool for ensuring clonality of cells."
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