GRIDs
— small chambers
made of liquids

GRIDs - small chambers made of liquids

GRIDs consist of indivudal liquid compartments. They can rapidly be applied to common polystyrene- and glass-bottom doshes, are scalable and extremely stable. GRIDs provide excellent optics on any microscope, operate with minute volumes and are easy to integrate into common workflows

How it works

GRIDs are held in place by interfacial tension. They are made by forming a very thin film of aqueous solution (e.g., cell-culture medium) onto common cell culture dishes, followed by overlaying the aqueous film with a second immiscible liquid. GRIDs are carved out of the aqueous phase and are bonded by liquid-liquid interfaces (and not solid walls)

A 16 x 16 GRID

The image displays a GRID consisting of 256 individual wells in a 60-mm dish. GRIDs are carved out of two immiscible liquid layers - an aqueouse phase at the bottom of the dish (e.g., cell culture media) and an immiscible overlay. Here, 16 different dyes have been added in all pair-wise combinations to mimic a combinatorial drug-screen.

Side-view of a GRID

The image displays a liquid GRID from the side with different amounts of aqueous red liquid added to individual wells. GRIDs are highly versatile structures, that can be applied to several applications - e.g., combinatorial drug screening, organism-based drug screens, single-cell biology and many more...

Key benefits and features

  • Cost-effective GRIDS can be applied onto common polystyrene and/or glass-bottom dishes. No need for costly upfront manufacturing
  • Save precious reagents GRIDS operate with nl-┬Ál volumes, and significantly reduce the consumption of precious reagents
  • Excellent optics GRIDS provide excellent optics on any microscope which is particularly useful for workflows applying single cells, such as cell-line development, sc-qRT-PCR, sc-WGA, as well as assuring the clonality of cells
  • Optimal and streamlined workflows GRIDS can easily be applied to dishes, removed from dishes and rebuild on demand - such flexibility and versatility is ideal for many workflows that require individual treatment (e.g., different drugs) of cells as well as common steps (e.g., fixation, washing)
  • Easy integration GRIDS easily integrate with downstream applications, such as sc-qRT-PCR, sc-WGA, or cell-cloning through automated seeding, feeding, washing and harvesting of cells

Testimonials

"For years we have worked with millilitre volumes, adding microliters of costly reagents because relatively large cell numbers (millions) were needed for our assays. This need no longer be the case, and we can now scale experiments down at least 3 orders of magnitude with the iota system. This becomes hugely important for academic groups working with expensive cells or reagents, how else could we afford to do a 256-well experiment in a single 60mm dish?"