GRIDs consist of indivudal liquid compartments. They can rapidly be applied to common polystyrene- and glass-bottom doshes, are scalable and extremely stable. GRIDs provide excellent optics on any microscope, operate with minute volumes and are easy to integrate into common workflows
GRIDs are held in place by interfacial tension. They are made by forming a very thin film of aqueous solution (e.g., cell-culture medium) onto common cell culture dishes, followed by overlaying the aqueous film with a second immiscible liquid. GRIDs are carved out of the aqueous phase and are bonded by liquid-liquid interfaces (and not solid walls)
"For years we have worked with millilitre volumes, adding microliters of costly reagents because relatively large cell numbers (millions) were needed for our assays. This need no longer be the case, and we can now scale experiments down at least 3 orders of magnitude with the iota system. This becomes hugely important for academic groups working with expensive cells or reagents, how else could we afford to do a 256-well experiment in a single 60mm dish?"